To understand the engineering of genetic material and thereby utilize DNA effectively.
CONTENTS
1. Isolation of genomic DNA from leaf samples - CTAB Precipitation method
2. Estimation of DNA / RNA by UV spectrophotometry
3. Restriction digestion of DNA
4. Construction of restriction map - plasmids
5. DNA ligation
6. Polymerase Chain Reaction - Amplification of DNA of interest/ RAPD
7. Purification of PCR products
8. Southern blotting / Western blotting / northern blotting
9. Cloning of PCR products (competent cell preparation, CaCl2 transformation, blue - white screening of transformants)
- ஆசிரியர்: Krishnakumar S