Handling of Instruments and Laboratory safety measures. 2. Handling and Maintenance of compound microscope. 3. Cleaning of Glassware and preparation of cleaning solutions. 4. Staining Procedures a. Smear preparation b. Simple staining c. Grams staining d. Capsule staining 5. Sterilization procedures and preparation of solid and liquid media 6. Streak plate method 7. Decimal dilution method 8. Spread plate method 9. Pour plate method 10. Antibiotic Sensitivity test