COURSE OBJECTIVES
Aimstoprovideabasicunderstandingofthecoreprinciplesandtopics ofBiochemistry
andtheirexperimentalbasis.
To enable to perform routine as well as special investigative procedures in different specialties
of Medical LaboratoryTechnology.
UNIT 1 Chemistry, metabolism & Clinical pathology of Carbohydrate 12 Hrs.
Chemistry of carbohydrates & their related metabolism - Introduction, definition, classification,
biomedical importance Brief outline of metabolism: Glycogenesis & glycogenolysis (in brief), Glycolysis,
citric acid cycle & its significance, HMP shunt & Gluconeogenesis, Diabetes mellitus, gestation diabetes
mellitus, Hyperglycemia & hypoglycemia and its causes.
UNIT 2 Chemistry of Amino acids and Protein- Protein Metabolism and its clinical pathology 12
Hrs.
Amino acids -Definition, classification, essential &non-essential amino acids. Chemistry of Proteins -
Introduction, definition, classification, structure, and its biomedical importance, Transformation,
Decarboxylation, Ammonia formation & transport, Urea cycle, metabolic disorders in urea cycle,
catabolism of amino acids especially Phenylalanine, Tyrosine & Tryptophan, Creatinine, Proteinuria.
UNIT 3 Chemistry of Lipids, Lipids metabolism & clinical Pathology 12 Hrs.
Introduction,definition, classification, biomedical importance, essential fatty acids. Non-essential fatty
acids, Brief out line of metabolism: Beta oxidation of fatty acids, fatty liver, Ketosis, Cholesterol & it's
clinical significance, Lipoproteins in the blood composition & their functions in brief, Atherosclerosis
UNIT 4 Enzymes and its clinical significance 12 Hrs.
Introduction, definition, classification, coenzymes, isoenzymes, properties, factors affecting enzyme
action, enzyme inhibition, diagnostic value of serum enzymes - Creatinine kinase, Alkaline
phosphatase, Acid phosphatase, LDH, SGOT, SGPT, Amylase, Lipase, Carbonic anhydrase etc
UNIT 5 Acid base balance concepts & disorders 12 Hrs.
Water metabolism, Distribution of fluids in the body, ECF, ICF, dehydration. - pH, Buffers, Acidosis,
Alkalosis
Max. 60 Hrs.
COURSE OUTCOMES
On completion of the course, students will be able to know
CO1 - Attain knowledge about the basics of biochemistry and the its importance.
CO2 - Attain knowledge on carbohydrates, proteins, fats and their functions in the body.
CO3 - Attain knowledge on the basic principles of metabolism and its regulation
CO4 - Understand the role of enzymes and its importance in diagnosis of various diseases.
CO5 - Understand the role of pH, buffer system and water balance in the body.
CO6 - Understand and access various
biochemical parameters and its relation to disease and dysfunction of organs.
- Teacher: James John