Sathyabama LMS

Course Objectives:

 ·        To learn the basic histological and cytological procedures

·        To understand the diagnostic applications of histological and cytological methods

 List of Experiments

1.         Hematoxylin and Eosin staining

2.         PAP staining

3.         Embedding

4.         Microtome: Uses, care, and parts

5.         PAS stain

6.         Pearls stain

7.         Reticulin stain

8.         Giemsa stain

Course Outcomes

Upon completion of the course, students will be able to

CO1 Demonstrate proficiency and expertise in the proper use of the light microscope in examining histological specimens on glass slides

CO2 Understand the basic concepts of tissue fixation, dehydration, embedding, sectioning, staining, and mounting of slides for histological examination, immunofluorescent staining, and electron microscopy

CO3 Differentiate the characteristics of tissues of the body (epithelium, connective, muscle, nerve) and their relationships in the various organ systems of the human body

CO4 Identify the histological features of selected tissues/organ systems resulting from disease processes

CO5 Examine how certain diseases can be diagnosed using histological and cytological methods

CO6 Demonstrate common histology procedures such as embedding tissue in paraffin, tissue sectioning, and mounting, or routine staining of tissue sections

SATHAYBAMA INSTITUTE OF SCIENCE AND TECHNOLOGY SCHOOL OF ALLIED HEALTH SCIENCES

DEPARTMENT OF MEDICAL LABORATORY TECHNOLOGY REGULATIONS 2023

SAMB2501

ANALYTICAL TECHNIQUES &

BIOINSTRUMENTATION-LAB

L T P Credits Total Marks

0 0 4 2 100

COURSE OBJECTIVES

Acquire practical knowledge about laboratory instrumentations.

Acquire knowledge about applications of centrifugation and electrophoretic methods in

laboratory.

Demonstrate the use of spectroscopic techniques.

Attain knowledge to use chromatographic techniques in research.

Apply molecular techniques in analysis & research.

LIST OF EXPERIMENTS

1. Centrifugation techniques Lab-Separation of blood WBC using fycoll reagent.

2. Chromatographic techniques Lab Demonstration of Paper chromatography.

3. Electrophoretic techniques- Demonstration of Agarose gel electrophoresis.

4. Electrophoretic techniques-Immunoelectrophoretic in agarose gel to see Ag-Ab reactions.

5. Molecular techniques- Demonstration of DNA extraction and quality check.

COURSE OUTCOMES

On completion of the course, students will be able to know

CO1 - Understand the basic instrumentation and their use in laboratory.

CO2 - Identify instruments and their uses.

CO3 - Recognize the types of separation techniques and their uses in lab diagnosis.

CO4 - Apply the practical knowledge and understanding to usage of instruments.

CO5 - Bridge the gap between clinical and theory during practice of instruments

CO6 - To acquire knowledge about various laboratory techniques

Course Objectives:
• To be able to fix, process, embed tissues and make sections for micro section studies
• To be competent to make routine cytological preparation

Course Outcomes:
On completion of the course, student will be able to
CO1 Identify the basic structure of cells, tissues and organs and describe their contribution to normal function.
CO2 Interpret light- and electron-microscopic histologic images and identify the tissue source and structures.
CO3 Demonstrate common histology procedures such as embedding tissue in paraffin, tissue sectioning and
mounting, or routine staining of tissue sections
CO4 Describe common histology laboratory procedures used to prepare stained slides from tissue samples.
CO5 Outline the principles of histochemistry, and types of microscopies utilized in histology
CO6 Understand professional procedures in museum display, collections, care and preservation