Sathyabama LMS

Course Objectives:

 ·        To learn the basic histological and cytological procedures

·        To understand the diagnostic applications of histological and cytological methods

 List of Experiments

1.         Hematoxylin and Eosin staining

2.         PAP staining

3.         Embedding

4.         Microtome: Uses, care, and parts

5.         PAS stain

6.         Pearls stain

7.         Reticulin stain

8.         Giemsa stain

Course Outcomes

Upon completion of the course, students will be able to

CO1 Demonstrate proficiency and expertise in the proper use of the light microscope in examining histological specimens on glass slides

CO2 Understand the basic concepts of tissue fixation, dehydration, embedding, sectioning, staining, and mounting of slides for histological examination, immunofluorescent staining, and electron microscopy

CO3 Differentiate the characteristics of tissues of the body (epithelium, connective, muscle, nerve) and their relationships in the various organ systems of the human body

CO4 Identify the histological features of selected tissues/organ systems resulting from disease processes

CO5 Examine how certain diseases can be diagnosed using histological and cytological methods

CO6 Demonstrate common histology procedures such as embedding tissue in paraffin, tissue sectioning, and mounting, or routine staining of tissue sections

Course Objectives:
• To understand the basic quality laboratory processes
• To apply the quality control tools

1.     Demonstration of various methods of quality control- Preparation of Quality control charts, a) Levy- Jennings and b) Cusum charts

2.     Demonstration of various methods of quality control- Westgard Rules to verify trends, biases, or errors in quality controls

3.     Sources of error, variation, and their correction methods, CAPA - corrective action & preventive action, Intrinsic and extrinsic and random errors

4.     Centrifugation techniques Basic principles, procedure, and working mechanism centrifugation techniques (zonal, differential, density gradient, and isopycnic centrifugation)

Lab-Separation of blood WBC using fycoll reagent

5.     Chromatographic techniques- Principles and working procedures of various chromatographic techniques (Column, ion exchange, Gas –liquid, affinity, molecular- exclusion, thin layer, adsorption, and partition)

Lab Demonstration of Paper chromatography

6.   Electrophoretic techniques- Principles and working procedures of various electrophoresis (Gel electrophoresis (vertical, horizontal)- polyacrylamide gel electrophoresis (PAGE)-SDS and isoelectric focussing)

Lab- Demonstration of gel electrophoresis

7.   Molecular techniques Isolation of DNA, RNA, Plasmids, and protoplast

Lab- Demonstration of DNA extraction and quality check.

8.     Principle and QC procedure for lab instruments- parts, principle, maintenance, and care of microscope. microscope, incubator, Hot air oven, Autoclave, Centrifuge, Colorimeter, Spectrophotometer, Flame photometer, Refrigerator, pH meter